Reverse transcription loop‑mediated isothermal amplification has a high performance in the detection of SARS‑CoV‑2 in saliva samples and nasal swabs from asymptomatic and symptomatic individuals

The detection of coronavirus disease 2019 cases represents a significant challenge at the epidemiological level. Limitations exist in effectively detecting asymptomatic cases, achieving good follow‑up hospitals without infrastructure for reverse transcription‑quantitative PCR (RT‑qPCR) or difficult‑to‑access areas and developing methods with need less invasive sampling procedures. Therefore, present study evaluated performance direct transcription loop‑mediated isothermal amplification (RT‑LAMP) test severe acute respiratory syndrome 2 (SARS‑CoV‑2) saliva nasal samples individuals belonging to university population. In addition, this was also assessed effectiveness symptomatic referred from hospital poor molecular biology located outside urban area. RT‑LAMP assay compared results obtained RT‑qPCR nasopharyngeal swab test, where diagnosis confirmed by lateral flow immunoassay rapid antigen detection. A total 128 were analyzed, which 43% positive individuals, 25% 32% SARS‑CoV2‑negative control individuals. Among no differences found between Cq values determined RT‑qPCR. sensitivity 96.5% specificity 97.6% reported SARS‑CoV‑2 salivary RT‑LAMP, as well 100% These findings indicated that using has high specificity, turn suggest it is viable reliable alternative use monitoring.